Center of Evolutionary Applications

Center of Evolutionary Applications (CEA) is a service unit based in the Department of Biology. We provide technical support for researchers at the Department, and offer genetic research and analysis services for external customers.

We are specialised in applied ecological and evolutionary genetics research. We carry out projects together with researchers from the Biology Department and also external collaborators from other universities and research institutes.

Our services include DNA/RNA extractions, microsatellite analysis, RAD-seq, qPCR, environmental DNA analysis (eDNA), DNA library preparation, metagenomics, Sanger and next-gen sequencing and methodological optimizations.

Current projects

Genotyping of White tailed eagle (University of Turku)

In this project we will do microsatellite genotyping of White tailed eagle samples.

 

Penttinen, I., Nebel, C., Stjernberg, T. et al. Large-scale genotypic identification reveals density-dependent natal dispersal patterns in an elusive bird of prey. Mov Ecol 12, 16 (2024). DOI: 10.1186/s40462-023-00447-5

Molecular analysis for sex identification (DISRUPT, University of Turku)

Using DNA based methods we can determine the sex of individuals in cases where no apparent visual differences are present. Here we use molecular methods to determine the sex of juvenile eider ducks by taking a swab sample. Egg shells can also be used as starting material for DNA analysis if the ducklings left the nest before sampling!

DISRUPT: Ducks as models for assessIng endocrine DISRUPTing chemicals in the aquatic environment

https://sites.utu.fi/disrupt/

DNA analysis for microbial studies (EXPEVO, University of Turku)

DNA sequencing is a powerful tool for species identification in microbial communities. The Experimental Evolution-project studies community dynamics, using microbes and an experimental approach as a means to study interactions both on ecological and evolutionary scales.

https://www.expevo.org/

Genotyping of Tawny owls (University of Turku)

In this project we do microsatellite genotyping of Tawny owls from different European populations.

DNA and RNA analysis for ticks (University of Turku/Metsähallitus)

We perform DNA and RNA extractions and determine pathogens from ticks as part of CoastNet LIFE project.

https://www.metsa.fi/en/project/coastnet-life/

DNA analysis for water mites (University of Turku)

We perform DNA analysis and species identification for water mites.

DNA analysis for soil microbes of Lupinus polyphyllus (University of Turku)

We perform DNA analysis and NGS library preparation for soil bacterial communities of Lupinus polyphyllus.

 

Mousavi, S. A., & Ramula, S. (2024). The invasive legume Lupinus polyphyllus has minor site-specific impacts on the composition of soil bacterial communities. Ecology and Evolution, 14, e11030. DOI: 10.1002/ece3.11030

DNA analysis for aquatic microbes (University of Turku)

Microbe DNA analysis for sea water filter samples and HMW DNA extraction for fungal parasite of phytoplankton for whole genome sequencing.

Phytoplankton-Parasite interactions along a salinity gradient

https://uglylab.science/

Past projects

RAD-tag genotyping of fish species (Swedish University of Agricultural Sciences)

In this project we will test and optimize a RAD-tag method for two fish species, pike and perch.

DNA analysis on Iijoki brown trout (ELY-centre North Ostrobothnia)

In this project we will use microsatellite genotyping for determining population structure in Iijoki brown trout.

Pilot study for testing eDNA method in determining fish abundance (Natural resources institute LUKE)

In this pilot study we will test an eDNA based method to determine the amount of brown trout individuals from water samples.

DNA and RNA analyses for the endophyte project (University of Turku)

In this project we will carry out DNA- and RNA extractions for the endophyte project.

https://blogit.utu.fi/helandersaikkonenlab/

Preparing metagenomic DNA libraries for Aero-DNA project (University of Turku)

In this pilot study we will extract DNA from pollen samples gathered by a Burkard trap. Sequencing a part of the "plant barcoding" gene trnL we can study the species composition in the samples.

Genetic analyses for the Metsänrajapuutarha-project (University of Turku)

More information here (in Finnish):

https://www.utu.fi/fi/yliopisto/luonnontieteiden-ja-tekniikan-tiedekunta/biologia/tutkimus

Wolf DNA analysis from non-invasive samples (Natural resources institute LUKE)

DNA based fingerprinting of wolf individuals is used for tracking individual's movements and supporting census counts of the Finnish wolf population by LUKE.

For more information see (in Finnish):

riistahavainnot.fi/suurpedot/dna

http://www.suurpedot.fi/suojelu-ja-metsastys/tutkimus/susien-dna-tutkimus.html

Blue tit genotyping (University of Turku)

For more info see:

https://www.utu.fi/en/units/sci/units/biology/research/projects/verg/Pages/JonBrommer.aspx

https://www.utu.fi/en/units/sci/units/biology/research/projects/verg/Pages/BarbaraClass.aspx

Genotyping Guadua bamboo species (University of Turku)

In this project we will use microsatellite genotyping for a population structure study.

DNA analysis for Idotea baltica, a littoral grazer (INVASIVES, University of Turku)

Introduced species can have wide ranging effects on the native species in the littoral ecosystem of the Baltic Sea. The project INVASIVES investigates how invasive species change community dynamics and reshape natural selection on the native species. Selective pressure alters patterns of variation in the genome, and these changes can be studied by sequencing.

https://sites.utu.fi/alga/

DNA library preparation for ticks (University of Turku)

Alale, T. Y., Sormunen, J. J., Vesterinen, E. J., Klemola, T., Knott, K. E., & Baltazar-Soares, M. (2024). Genomic signatures of hybridization between Ixodes ricinus and Ixodes persulcatus in natural populationsEcology and evolution14(5), e11415. DOI: 10.1002/ece3.11415

Alumns

Laura Karunen, summer trainee

Titiksha Peetumber, summer trainee & research assistant

Telma Kuuslampi, summer trainee & research assistant

Janina Karttunen, summer trainee & research assistant

Natalie Tomnikov, summer trainee & research assistant

Contact information

Contact us: cea@utu.fi

Visit us: University Hill, Natura building, 3. floor, room 333 and 342

Delivery address: Vesilinnantie 5, 20500 TURKU