Medisiina Imaging Centre
Medisiina Imaging Centre in the Medisiina C2 provides selection of modern bioimaging devices. Instrument reservations are done via OpenIRIS online reservation system. User training is mandatory.
Main features:
- Objectives: 5x, 10x, 20x, 20x long working distance, 40x long working distance, 63x oil
- Channels: 405 nm, 488 nm, 561 nm, 647 nm
- Holders: slides, 35 mm dishes, wellplates
- Live imaging chamber (temperature and CO2 control)
- Sensitive camera for low light live cell imaging
Contact: Markus Peurla
Laser capture microdissection is used to cut the areas of interest from tissue sections for different downstream molecular analyses.
Main features:
- Ultraviolet laser (UV) cutting and infrared laser capture (IR)
- Sample types: FFPE, frozen sections, cell cultures
- Area identification by brightfield or fluorescence staining
Contact: Markus Peurla
3DHistech Pannoramic slide scanners are used to automatically image whole tissue section slides stained with brightfield or fluorescence stains.
Contact: Markus Peurla
Instruments:
Pannoramic P1000 slide scanner
- Brightfield
- Slide formats: 25 mm x 75 mm and macro slide (50 mm x 75 mm)
- 20x/0.8 and 40x/0.95 objectives
- Capacity: 600 normal slides and 200 macro slides
- Speed: 10-40 slides per hour
Pannoramic Midi fluorescence slide scanner
Fluorescence and brightfield
20x/0.8 and 40/0.95 objectives
Fluorescence filters:
- Dapi: Zeiss Filter Set 49
- Fitch: Zeiss Filter Set 38
- Rhodamine/TRITCH: Zeiss Filter Set 43HE
- DAPI/FITC/TRITC/Cy5: Semrock multiband filter
Capacity: normal slides (25 mm x 75 xx)
Speed 1-5 slides per hour
Pannoramic P250 slide scanner
- Brightfield
- Objective 20x/0.8
- Capacity: 250 normal slides (25 mm x 75 xx)
- Speed: 10-20 slides per hour
Slide scanner output file format is Mirax (mrxs). Install the free CaseViewer for viewing images.
Azure Sapphire Biomolecular Imager
The Sapphire Biomolecular RGBNIR Imager is a next-generation laser scanning system that provides exceptional data quality through extremely sensitive detection, ultra high resolution, and broad linear dynamic range. The Sapphire is capable of fluorescence, chemiluminescence, and phosphorimaging. Fluorescence excitation (nm): 488, 520, 658 and 784.
Fuji BAS-5000
Fuji Analyzer BAS-5000 is a phosphorimager that scans autoradiography plates. It can be used for scanning the image stored on special autoradiography plates that store energy (for example from radioactivity (beta, gamma)) into small crystals on the plate from ex vivo, in vitro, etc. studies. This scanner can reach up to 25 micrometer resolution. The BAS-5000 is no longer manufactured and newer phosphorimagers do not achieve the same scan speed, sensitivity and other characteristics anymore.
Zeiss AxioImager M1 is an upright epifluorescence microscope with transmitted light (phase and dark field) and motorized stage. Image acquisition through Zeiss AxioCam MRm (monochrome) camera or Zeiss AxioCam 506 color camera and Zen software.
- Objectives: 5x, 10x, 20x, 40x oil, 63x oil
- Channels: DAPI, GFP, Rhodamine, Alexa Fluor 660