The N2-fixing cyanobacterium Anabaena sp. PCC7120 is a promising bioproduction platform for concomitant reductive biotransformation for value chemical generation in vegetative cells and production of hydrogen in heterocysts. A promising starting point is the deletion of Flavodiiron 3A in vegetative cells, which is part of the auxiliary electron transport chain and subtracts electrons from reductive biotransformations. This deletion also leads to increased H2 production in the heterocysts. The underlying mechanisms for this as well as the roles of Flv2 and Flv4 are to be evaluated. Moreover, a heterologous oxidoreductase is to be introduced for value chemical production. To increase product yield and facilitate downstream processing, the mutant strain is to be immobilized. Investigation of photosynthesis, metabolism and regulatory networks of the mutant strains is  performed using -omics, single reaction monitoring, BiFC combined with FRET, Membrane inlet mass spectrometry (MIMS) and modelling approaches.